Favre-Godal, Quentin and Pinto, Sébastien and Dorsaz, Stephane and Rutz, Adriano and Marcourt, Laurence and Gupta, Mahabir and Sanglard, Dominique and Queiroz, Emerson F. and Wolfender, Jean-Luc (2018) Identification of antifungal compounds from the Root Bark of Cordia anisophylla J.S. Mill. Sociedade Brasileira de Química, 00 (00). pp. 1-7.
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Abstract
The dichloromethane extract of the root bark of the Panamanian plant Cordia anisophylla J.S. Mill. (Boraginaceae) presented antifungal activity against a susceptible strain of Candida albicans in a bioautography primary screening. The susceptible strain was used to detect minor active compounds that would not have been detected using a classical approach. In order to identify the antimicrobial compounds, the active extract was fractionated by semi-preparative high-performance liquid chromatography and the fractions were submitted to the antifungal bioassay. This procedure enabled a precise localization of the antifungal compounds directly in the chromatogram of the crude extract and allowed for an efficient, targeted isolation. Four compounds were isolated, one of which is a new natural product. The structures were elucidated using spectroscopic methods. Their antifungal properties were evaluated by determination of the minimum inhibitory quantity and concentration by bioautography and dilution assay against a wild type strain of C. albicans.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cordia anisophylla, candida albicans, susceptible strain, antifungal, HPLC-microfractionation |
| Subjects: | Q Science > QD Chemistry Q Science > QK Botany S Agriculture > SB Plant culture |
| Depositing User: | Fergie Pineda |
| Date Deposited: | 02 Nov 2023 03:06 |
| Last Modified: | 02 Nov 2023 03:06 |
| URI: | http://up-rid.up.ac.pa/id/eprint/6892 |
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